CHARACTERIZATION MORPHOLOGICAL, CULTURAL AND PATHOGENIC OF ISOLATED colletotrichum sp. ANTHRACNOSE PRODUCING IN MANGO (mangifera indica L.).
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Published:
2017-09-01
Keywords:
Glomerella cingulata, anthracnose, appressoria, conidia pathogenicity.
Main Article Content
Abstract
Anthracnose is considered the main fungal disease affects mango. Cause considerable damage to leave, flowers and
fruits. The filamentous fungi of the genus Colletotrichum and its teleomorph Glomerella pathogens are considered the
most widely distributed plants worldwide, causing this disease. In this investigation were obtained 22 isolates of Colletotrichum
from different localities of the country western region, in six varieties of mango. Cultural characterization
was varied in the growth of colonies on PDA, with presence and absence of exudation at different temperatures, 24
degrees being optimal for growth. The observed in isolated sexual stage shows few perithecia. Conidia concentration
ranged between 107 and 105 measured Neubauer chamber while appresoria and conidia are within the ranges for the
species Colletotrichum gloeosporioides. Variability in pathogenicity showed that all species investigated wizards are susceptible
to microorganism. The statistical analysis shows the grouping of isolates by locality with similar pathogenic
characteristics.
fruits. The filamentous fungi of the genus Colletotrichum and its teleomorph Glomerella pathogens are considered the
most widely distributed plants worldwide, causing this disease. In this investigation were obtained 22 isolates of Colletotrichum
from different localities of the country western region, in six varieties of mango. Cultural characterization
was varied in the growth of colonies on PDA, with presence and absence of exudation at different temperatures, 24
degrees being optimal for growth. The observed in isolated sexual stage shows few perithecia. Conidia concentration
ranged between 107 and 105 measured Neubauer chamber while appresoria and conidia are within the ranges for the
species Colletotrichum gloeosporioides. Variability in pathogenicity showed that all species investigated wizards are susceptible
to microorganism. The statistical analysis shows the grouping of isolates by locality with similar pathogenic
characteristics.
Article Details
Section
Scientific Article
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References
Abd-AllA M and Wafaa M. 2010. New Safe Methods for Controlling Anthracnose Disease of Mango (Mangifera indica L.) Fruits Caused by Colletotrichum gloeosporioides (Penz.). Journal of American Science 8(8).
Alvarez, E., J. Mejía, G. Llano y J. Loke. 2006. Characterization of Colletotrichum gloeosporioides, causal agent of anthracnose in soursop (Annona muricata) in Valle del Cauca. Poster: International Center for Tropical Agriculture (CIAT).
Arauz, LF. 2000. Mango anthracnose: Economic impact and current options for integrated management. Plant Disease. 84:600–611.
Bruwer, T., le Legadec, M.D. y Köhne, J.S.2006. A Decade of Mango Cultivar Evaluation by Westfalia Technological Services. 8th International Mango Symposium. South Africa Feb. 5-10: 113-118.
Butt T and Copping L. 2000. Fungal biological control agents. Pesticide Outlook 11: 186-191.
Campbell, R.J. 1992. A Guide to Mangos in Florida. Fairchild Tropical Garden, Miami, Florida. E.E. U.U.
Cañizares, J.1966. El Mango. INRA. La Habana. Cuba: 6-14: 42-44.
Capote, M., Lima, H., Cabrera, A.M., Naviera, A. y Blanco, M. 1989. Agrupación fenotípica de 31 cultivares de mango sobre la base de variables morfológicas y de resistencia a enfermedades. Cienc. Tec. Agric. Cítricos y otros frutales. 12 (4): 12-18.
Carrillo J A, García R, Muy M D, Sañudo A, Márquez I, Allende R, de la Garza Z, Patiño M, Galindo E. 2005. Control biológico de la antracnosis (Colletotrichum gloeosporioides (Penz) Penz & Sacc) y su efecto en la calidad poscosecha del mango (Mangífera índica L) en Sinaloa, México. Rev. Mexicana de Fitopatología 23 (001): 24-32.
Cerón, L., B. Higuera, J. Sánchez, S. Bustamante y G. Buitrago. 2007. Crecimiento y desarrollo de Colletotrichum gloeosporioides f. alatae durante su cultivo en medios líquidos. Acta Biológica Colombiana, 11(1): 99–109.
Cigarroa, A. 1985. Biometría y Diseño Experimental. La Habana. Editorial Pueblo y Educación (743).
Denoyes B, Guérin G, Délye C. 2003. Genetic diversity and pathogenic variability among isolates of Colletotrichum species from strawberry. Phytopathology 93: 219–28.
Freeman, S., Katant, T. and Shabi, E. 1998. Characterization of Collectotrichum species responsible for anthracnose disease of various fruits. Plant Disease. 82 (6): 596-605.
Gutiérrez J, Nieto D, Téliz D, Zavaleta E, Vaquera H, Martínez T, Delgadillo F. 2001. Características del crecimiento, esporulación y patogenicidad de aislamientos de Colletotrichum gloeosporioides (Penz) obtenidos de frutos de mango (Mangífera índica L). Rev. Mexicana de Fitopatología 19 (001): 90-93.
Infoagro. 2006. El mercado de las frutas tropicales en la unión europea. http//www.infoagro.com/España. 10pp.
Jayasinghe, C., y Fernando T. 2009. First Report of Colletotrichum acutatum on Mangifera indica L in Sri Lanka. Ceylon Journal of Science (Biological Sciences). 38 (1).
Kuo, K.C. 1999. Germination and appressorium formation in Colletotrichum gloeosporioides. Proc. Natl. Sci. Counc. ROC (B). 23 (3): 126-132.
Páez, A.R. 1996. Respuesta de variedades de mango (Mangífera índica L) a la antracnosis (Colletotrichum gloeosporioides). Fitopatología Colombiana. 20 (1): 11-19.
Rebouca, A. 2002. Aspectos sobre la producción de mango. Curso Internacional de mango. Ciudad de Guatemala. Guatemala: 20-23.
Rodríguez, M., Guerrero, M., Sandoval, R. 2002. Cultivo del mango. Guía Técnica. Centro Nacional de Tecnología Agropecuaria y Forestal (12).
Sanders, G., Korsten L and Wehner F. 2000. Market survey of post harvest diseases and incidence of Colletotrichum gloeosporioides on avocado and mango fruit in South Africa. Trop. Sci., 40(4): 192-198.
Sangeetha, C.G, and Rawal.R.D. 2008. Nutritional Studies of Colletotrichum gloeosporioides (Penz.) Penz. and Sacc. The Incitant of Mango Anthracnose. World Journal of Agricultural Sciences 4 (6): 717-720.
Santiago, K., Hojo, T., Lacerda, O., Pereira, M., Alves, A., Luna, G., Prado, A., Rebouças são, A., Oliveira, N., Macedo, G. 2005. Patogenicidade causada pelo fungo Colletotrichum gloeosporioides (Penz) em diferentes espécies frutíferas. Rev. Bras. Frutic., Jaboticabal – Sao Pablo. 28 (1): 131-133.
Sutton, B.C. 1980. The Coelomycetes. Fungi Imperfecti with Pycnidia Acervuli and Stromata. Commonwealth Mycological Institute. Assoc. Appl. Biol., Kew, Surrey, England.
Torres M. 1995. Resultados obtenidos en las investigaciones. Estación Nacional de Frutales. MINAGRI: 18-29.
Valdés, L., A. Gómez, M. Carballo, M. Capote, I. González y W. Rohde. 2015. Estandarización de protocolos para la extracción de ADN cromosómico en cepas de Colletotrichum gloeosporioides aislados en plantas de mango (Mangifera indica L.). La Granja: Revista de Ciencias de la Vida. Vol. 22(2): 40-49. ISSNp: 1390-3799, ISSNe: 1390-8596.
Alvarez, E., J. Mejía, G. Llano y J. Loke. 2006. Characterization of Colletotrichum gloeosporioides, causal agent of anthracnose in soursop (Annona muricata) in Valle del Cauca. Poster: International Center for Tropical Agriculture (CIAT).
Arauz, LF. 2000. Mango anthracnose: Economic impact and current options for integrated management. Plant Disease. 84:600–611.
Bruwer, T., le Legadec, M.D. y Köhne, J.S.2006. A Decade of Mango Cultivar Evaluation by Westfalia Technological Services. 8th International Mango Symposium. South Africa Feb. 5-10: 113-118.
Butt T and Copping L. 2000. Fungal biological control agents. Pesticide Outlook 11: 186-191.
Campbell, R.J. 1992. A Guide to Mangos in Florida. Fairchild Tropical Garden, Miami, Florida. E.E. U.U.
Cañizares, J.1966. El Mango. INRA. La Habana. Cuba: 6-14: 42-44.
Capote, M., Lima, H., Cabrera, A.M., Naviera, A. y Blanco, M. 1989. Agrupación fenotípica de 31 cultivares de mango sobre la base de variables morfológicas y de resistencia a enfermedades. Cienc. Tec. Agric. Cítricos y otros frutales. 12 (4): 12-18.
Carrillo J A, García R, Muy M D, Sañudo A, Márquez I, Allende R, de la Garza Z, Patiño M, Galindo E. 2005. Control biológico de la antracnosis (Colletotrichum gloeosporioides (Penz) Penz & Sacc) y su efecto en la calidad poscosecha del mango (Mangífera índica L) en Sinaloa, México. Rev. Mexicana de Fitopatología 23 (001): 24-32.
Cerón, L., B. Higuera, J. Sánchez, S. Bustamante y G. Buitrago. 2007. Crecimiento y desarrollo de Colletotrichum gloeosporioides f. alatae durante su cultivo en medios líquidos. Acta Biológica Colombiana, 11(1): 99–109.
Cigarroa, A. 1985. Biometría y Diseño Experimental. La Habana. Editorial Pueblo y Educación (743).
Denoyes B, Guérin G, Délye C. 2003. Genetic diversity and pathogenic variability among isolates of Colletotrichum species from strawberry. Phytopathology 93: 219–28.
Freeman, S., Katant, T. and Shabi, E. 1998. Characterization of Collectotrichum species responsible for anthracnose disease of various fruits. Plant Disease. 82 (6): 596-605.
Gutiérrez J, Nieto D, Téliz D, Zavaleta E, Vaquera H, Martínez T, Delgadillo F. 2001. Características del crecimiento, esporulación y patogenicidad de aislamientos de Colletotrichum gloeosporioides (Penz) obtenidos de frutos de mango (Mangífera índica L). Rev. Mexicana de Fitopatología 19 (001): 90-93.
Infoagro. 2006. El mercado de las frutas tropicales en la unión europea. http//www.infoagro.com/España. 10pp.
Jayasinghe, C., y Fernando T. 2009. First Report of Colletotrichum acutatum on Mangifera indica L in Sri Lanka. Ceylon Journal of Science (Biological Sciences). 38 (1).
Kuo, K.C. 1999. Germination and appressorium formation in Colletotrichum gloeosporioides. Proc. Natl. Sci. Counc. ROC (B). 23 (3): 126-132.
Páez, A.R. 1996. Respuesta de variedades de mango (Mangífera índica L) a la antracnosis (Colletotrichum gloeosporioides). Fitopatología Colombiana. 20 (1): 11-19.
Rebouca, A. 2002. Aspectos sobre la producción de mango. Curso Internacional de mango. Ciudad de Guatemala. Guatemala: 20-23.
Rodríguez, M., Guerrero, M., Sandoval, R. 2002. Cultivo del mango. Guía Técnica. Centro Nacional de Tecnología Agropecuaria y Forestal (12).
Sanders, G., Korsten L and Wehner F. 2000. Market survey of post harvest diseases and incidence of Colletotrichum gloeosporioides on avocado and mango fruit in South Africa. Trop. Sci., 40(4): 192-198.
Sangeetha, C.G, and Rawal.R.D. 2008. Nutritional Studies of Colletotrichum gloeosporioides (Penz.) Penz. and Sacc. The Incitant of Mango Anthracnose. World Journal of Agricultural Sciences 4 (6): 717-720.
Santiago, K., Hojo, T., Lacerda, O., Pereira, M., Alves, A., Luna, G., Prado, A., Rebouças são, A., Oliveira, N., Macedo, G. 2005. Patogenicidade causada pelo fungo Colletotrichum gloeosporioides (Penz) em diferentes espécies frutíferas. Rev. Bras. Frutic., Jaboticabal – Sao Pablo. 28 (1): 131-133.
Sutton, B.C. 1980. The Coelomycetes. Fungi Imperfecti with Pycnidia Acervuli and Stromata. Commonwealth Mycological Institute. Assoc. Appl. Biol., Kew, Surrey, England.
Torres M. 1995. Resultados obtenidos en las investigaciones. Estación Nacional de Frutales. MINAGRI: 18-29.
Valdés, L., A. Gómez, M. Carballo, M. Capote, I. González y W. Rohde. 2015. Estandarización de protocolos para la extracción de ADN cromosómico en cepas de Colletotrichum gloeosporioides aislados en plantas de mango (Mangifera indica L.). La Granja: Revista de Ciencias de la Vida. Vol. 22(2): 40-49. ISSNp: 1390-3799, ISSNe: 1390-8596.